recombinant human rh tgfβ  (R&D Systems)

Journal: iScience

Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

doi: 10.1016/j.isci.2024.109909

Figure Lengend Snippet:

Article Snippet: recombinant human (rh) TGFβ , Peprotech , Cat# 100-21A.

Techniques: Control, Recombinant, Modification, Cell Recovery, Lysis, Reverse Transcription, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Software, Microscopy

Journal: iScience

Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

doi: 10.1016/j.isci.2024.109909

Figure Lengend Snippet: Inflammatory cytokines are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also Figures S2–S4 .

Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

Techniques: Gene Expression, Expressing, In Vitro, Activation Assay, Inhibition, Quantitative RT-PCR

Journal: iScience

Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

doi: 10.1016/j.isci.2024.109909

Figure Lengend Snippet: IFNγ and cytokine mix stimulation increases IAP activity and FABP6 protein expression in HFOs (A and B) Alkaline phosphatase activity level (μg pNPP/min) in (A) proximal and (B) distal HFOs upon cytokines stimulation ( n = 9 independent donors). (C) Alkaline phosphatase (IAP) staining of embedded HFOs upon IFNγ, and cytokine mix stimulation compared to control. (D) FABP6 protein expression measured by western blot in distal HFOs. (E) FABP6 western blot quantification on n = 5 independent donors. ∗ p < 0.05, ∗∗ p < 0.01 as determined by non-parametric one-way ANOVA.

Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

Techniques: Activity Assay, Expressing, Staining, Control, Western Blot

Journal: Pathophysiology

Article Title: The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation

doi: 10.3390/pathophysiology29040050

Figure Lengend Snippet: Effect of TGFβ1 on adipocyte differentiation on undifferentiated preadipocytes and early commitment stage of adipocyte differentiation. TGFβ1 was added to cells at day 0. A representative picture at 100× magnification was taken on days 3, 6, and 10 after Oil Red O staining for TGFβ1 treatment on undifferentiated preadipocytes (Un-Diff) and early commitment stage of adipocyte differentiation (R-Diff).

Article Snippet: In order to study the effect of the TGFβ1 on different phases of adipocyte differentiation (preadipocyte, commitment, and terminal differentiation), recombinant active human (rh) TGFβ1 protein (2 ng/mL: Catalogue Number 240-B-010, R & D Systems, Minneapolis, MN, USA) was used.

Techniques: Staining

Journal: Pathophysiology

Article Title: The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation

doi: 10.3390/pathophysiology29040050

Figure Lengend Snippet: Effect of TGFβ1 on adipogenic ( A ), ECM remodeling ( B ) and inflammatory ( C ) markers on Preadipocytes (Un-Diff) and Commitment stage of adipocyte differentiation (R-Diff). The result is a representative result of 2 independent experiments. Data expressed as Mean ± SD. Two-way ANOVA with Sidak’s multiple comparison test used to compare the effect of TGFβ1 on Un-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, † vs. R-Diff. ‡ vs. Un-Diff + TGFβ1.

Article Snippet: In order to study the effect of the TGFβ1 on different phases of adipocyte differentiation (preadipocyte, commitment, and terminal differentiation), recombinant active human (rh) TGFβ1 protein (2 ng/mL: Catalogue Number 240-B-010, R & D Systems, Minneapolis, MN, USA) was used.

Techniques: Comparison

Journal: Pathophysiology

Article Title: The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation

doi: 10.3390/pathophysiology29040050

Figure Lengend Snippet: Effect of TGFβ1 on adipogenic ( A ), ECM remodeling ( B ), and inflammatory ( C ) markers on Terminal differentiated adipocytes with S-diff and R-Diff. The result is a representative result of 3 independent experiments. Data expressed as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to compare Un-Diff, S-Diff, and R-Diff. Two-way ANOVA with Sidak’s multiple comparison test was used to compare the effect of TGFβ1 on S-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, † vs. S-Diff. ‡ vs. R-Diff, § vs. S-Diff + TGFβ1.

Article Snippet: In order to study the effect of the TGFβ1 on different phases of adipocyte differentiation (preadipocyte, commitment, and terminal differentiation), recombinant active human (rh) TGFβ1 protein (2 ng/mL: Catalogue Number 240-B-010, R & D Systems, Minneapolis, MN, USA) was used.

Techniques: Comparison

Journal: Pathophysiology

Article Title: The Effect of TGFβ1 in Adipocyte on Inflammatory and Fibrotic Markers at Different Stages of Adipocyte Differentiation

doi: 10.3390/pathophysiology29040050

Figure Lengend Snippet: Effect of TGFβ1 on Collagen VI protein, ( A ) Commitment stage of adipocyte differentiation: The result is a representative result of 2 independent experiments. Data expressed as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to compare the effect of TGFβ1 on Un-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, vs. R-Diff. ( B ) Mature adipocytes: The result is a representative result of 3 independent experiments. Data expressed as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to compare Un-Diff, S-Diff, and R-Diff. Two-way ANOVA with Sidak’s multiple comparison test was used to compare the effect of TGFβ1 on S-Diff and R-Diff. p -value < 0.05 * vs. Un-Diff, vs. S-Diff. vs. R-Diff, vs. S-Diff + TGFβ1. The levels of collagen VI protein, visualized by Western blot, were normalized to total protein loading obtained from stain-free imaging and shown in the histogram.

Article Snippet: In order to study the effect of the TGFβ1 on different phases of adipocyte differentiation (preadipocyte, commitment, and terminal differentiation), recombinant active human (rh) TGFβ1 protein (2 ng/mL: Catalogue Number 240-B-010, R & D Systems, Minneapolis, MN, USA) was used.

Techniques: Comparison, Western Blot, Staining, Imaging